Leibovitz L-15 Medium With 2mM L-Glutamine, is supplied by HiMedia. It is available in the form of an orangish red coloured, clear solution. It has a pH in the range of 7.3 -7.9 and an osmolality between 300.00 -340.00 Osm/Kg H20. It is advisable to store this product between 2-8C and away from bright light. Media composition (in mg/L) is as follows:

Calcium chloride dihydrate (185.00)

Magnesium chloride hexahydrate (200.00)

Magnesium sulphate anhydrous (97.72)

Potassium chloride (400.00)

Potassium phosphate monobasic (60.00)

Sodium chloride (8000.00)

Sodium phosphate dibasic anhydrous (190.12)

Amino acids

Choline chloride (1.00)

D-Ca-Pantothenate (1.00)

Folic acid (1.00)

Nicotinamide (1.00)

Pyridoxine hydrochloride (1.00)

Riboflavin-5-phosphate sodium salt (0.10)

Thiamine monophosphate (1.00)

i-Inositol (2.00)

D-Galactose (900.00)

Phenol red sodium salt (11.00)

Sodium pyruvate (550.00)

Leibovitzs Medium was specifically designed to grow cells in a CO2 free atmosphere. The standard sodium bicarbonate/ CO2 buffering system is replaced by combination of free basic amino acids, phosphate buffers and higher levels of galactose and sodium pyruvate. As a result, the medium does not require supplementation with sodium bicarbonate and can be used under conditions of free gaseous exchange with the atmosphere. The medium can be used to grow human tumor cells and embryonic cells and also established cell lines like HeLa and Hep-2. The medium is frequently used in diagnostic virology where tissue cell lines or strains need to be grown in closed systems. Leibovitzs medium obviates the need of frequent medium change. This is a 1X Liquid Cell Culture Medium with mM L-glutamine. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.