Sterile Luria Bertani Broth is supplied by HiMedia. It is a sterile ready prepared media containing 10ml of yellow to amber coloured solution in bottles. Sterile Luria Bertani Broth is generally used for the cultivation and maintenance of recombinant strains of Escherichia coli and may also be used for routine cultivation of not particularly fastidious microorganisms. It is advisable to store this ready prepared media between 2-8C. Composition (in gm/L) is as follows:

Casein enzymic hydrolysate (10.00)

Yeast extract (5.00)

Sodium chloride (10.00)

Luria Bertani Broth, Miller contains double the amount of sodium chloride as compared to original media described by Lennox, which is used for cultivation and maintenance of recombinant strains of Escherichia coli. This media is nutritionally rich for the growth of pure cultures of recombinant strains. Strains derived from Escherichia coli K12 are deficient in Vitamin B synthesis, and are further modified by specific mutation to create auxotrophic strains. These are therefore unable to grow on nutritionally deficient media. Casein enzymic hydrolysate provides peptides and peptones while Vitamin B complex is provided by yeast extract. Sodium chloride provides sodium ions for membrane transport and also maintains the osmotic equilibrium of the medium.