Lysozyme has been used for lysing E. coli and Streptomycetes for extraction purposes such as extracting group specific antigen. It would appear that lysozyme may act as a germinative agent of bacterial spores.In humans, lysozyme may be the mediator in the anti-tumor function of macrophages which, it has been shown, secrete the enzyme. There is evidence that cartilage lysozyme has a role in cartilage calcification. Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts