McCoy's 5A medium is supplied by HiMedia. It is available in the form of an orangish red coloured clear solution which has a pH in the range of 7.0 to 7.6 and an osmolality between 300.00-340.00 Osm/Kg H20. It is advisable to store this product between 2-8C, away from bright light. The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures. This medium is also hybridoma tested.

Quantity provided: 5 x 100ml /EA

The first McCoys 5A medium was developed in 1955 as the result of studies on the nutritional requirements of the Walker 256 carcinoma. The original formulation was based on the amino acids in concentrations similar to those in Eagle's medium as well as water-soluble vitamins of Medium 199. Modifications to the original formulation resulted in the final version being published in 1960. The final formulation also incorporates modifications done by Iwakata and Grace and contains increased amounts of folic acid, vitamin B12, and peptone. This medium is also known to support growth of primary cultures derived from a variety of tissues. McCoys 5A medium contains L-Glutamine and sodium bicarbonate. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.