Minimum Salt w/ Casein Acid Hydrolysate is supplied by HiMedia. It is available in the form of a cream to yellow homogeneous free flowing powder with a pH in the range of 6.6 to 7.0. Minimum Salt w/ Casein Acid Hydrolysate is used for the cultivation of Escherichia coli strains used for genetic and molecular studies. It is advisable to store this product below 30C. Composition (in gm/L) is as follows:

Casein acid hydrolysate (4.00)

Disodium hydrogen phosphate (6.80)

Monopotassium hydrogen phosphate (3.00)

Sodium chloride (0.50)

Ammonium chloride (1.00)

Dextrose (4.00)

Magnesium sulphate (0.24)

Minimum Salt with Casein Acid Hydrolysate is prepared based on the formula suggested by Davis et al. This medium, with the addition of casein acid hydrolysate can be used for cultivating Escherichia coli strains, for genetic and molecular studies. Casein acid hydrolysate provides many amino acids (except tryptophan) to E. coli. Ammonium chloride is added as a nitrogen source. Dextrose serves as the carbon and energy source while the two phosphates buffer the medium against pH changes due to the utilization of carbohydrate. Magnesium ions are required in a variety of enzymatic reactions including DNA replication.