Sensitive substrate for continuous assays and in situ determination of the matrix metalloproteinase activity. When cleaved at the Gly-Leu bond it separates the highly fluorescent Mca group from the efficient 2,4-dinitrophenyl quencher and results in the increase of fluorescence intensity. The kcat/km value for the punctuated metalloproteinase (MMP-7) and for gelatinase (MMP-2), e.g. are 1.7-105 and 6.3-105 M-1s-1, respectively ; Tested Applications: HPLC; Molecular Weight: 1093.2 Da; Purity: Greater than 98 % purifiedConjugation: Unconjugated