Mueller Hinton Broth is supplied by HiMedia. It is a sterile ready prepared media containing light amber coloured solution in bottles. Mueller Hinton Broth is generally used to determine the susceptibility of bacteria to sulphonamides by the tube dilution method. Also used for primary isolation of gonococci and meningococci. It is advisable to store this ready prepared media between 2-8C. Composition (in gm/L) is as follows:

Beef, infusion from (300.00)

Casein acid hydrolysate (17.50)

Starch (1.50)

The Mueller Hinton formulation was originally developed as a simple, transparent agar medium for the cultivation of pathogenic Neisseria species. Other media were subsequently developed that replaced the use of Mueller Hinton Agar for the cultivation of pathogenic Neisseria species, but it became widely used in the determination of sulfonamide resistance of gonococci and other organisms. Beef infusion and casein acid hydrolysate provide nitrogenous compounds, carbon, sulphur and other essential nutrients. Starch acts as a protective colloid against toxic substances present in the medium. Starch hydrolysis yields dextrose, which serves as a source of energy. These ingredients are selected for low thymine and thymidine content as determined by MIC values for Enterococcus faecalis with sulfamethoxazoletrimethoprim (SXT).