NCTC 109 Medium With L-Glutamine Without Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 3.0 -3.6 whereas the pH with Sodium Bicarbonate is between 6.8 -7.4. The osmolality without Sodium Bicarbonate is in the range of 230.00 -270.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 280.00 -320.00 Osm/Kg H20. It is advisable to store this product between 2-8C. Media composition (in mg/L) is as follows:

Calcium chloride dihydrate (265.00)

Magnesium sulphate anhydrous (100.00)

Potassium chloride (400.00)

Sodium chloride (6800.00)

Sodium dihydrogen phosphate anhydrous (122.00)

Amino acids

Calciferol (0.25)

Choline chloride (1.25)

D-Biotin (0.02)

D-Pantothenic acid (hemicalcium) (0.02)

DL-Tocopherol phosphate disodium salt (0.02)

Folic acid (0.04)

L-Ascorbic acid (50.00)

Menadione sodium bisulphite (0.12)

Nicotinamide (0.062)

Nicotinic acid (0.062)

Pyridoxal hydrochloride 0.062)

Pyridoxine hydrochloride (0.062)

Retinol Acetate (0.25)

Riboflavin (0.02)

Thiamine hydrochloride (0.02)

Vitamin B12 (10.00)

myo-Inositol (0.12)

p-Amino benzoic acid (PABA) (0.12)

2' Deoxyadenosine (10.00)

2' Deoxycytidine hydrochloride (10.00)

2' Deoxyguanosine hydrochloride (10.00)

5'-Methylcytosine hydrochloride (0.10)

Cocarboxylase (1.00)

Coenzyme A sodium salt (2.50)

D-Glucosamine hydrochloride (3.85)

D-Glucose (1000.00)

D-Glucuronolactone (1.80)

Flavin Adenine Dinucleotide Disodium salt (1.00)

Glucuronate sodium salt (1.80)

Glutathione sodium salt (20.00)

L-Amino-n-Butyric acid (5.51)

L-Taurine (4.18)

Phenol red sodium salt (20.00)

Thymidine (10.00)

Tween 80 (12.50)

Uridine triphosphate sodium salt (1.00)

-NAD (7.00)

-NADP (1.00)

NCTC 109 is one of the chemically defined medium in the series on NCTC media developed by Virginia Evans of the Tissue Culture Section of National Cancer Institute, Bethesda. NCTC 109 was the result of many years of development and modifications. The medium was originally formulated to establish and maintain a strain of mouse cells, L929. The medium has been shown to support growth of fibroblast-like and epithelial- like cells of both normal and malignant origin from mice, hamsters, monkeys and humans. NCTC 135 is similar to NCTC 109 except that L-Cysteine has been replaced with L-Cystine due to possible side effects of L-Cysteine on certain cell lines.