New Born Calf Serum, Heat inactivated,Sterile filtered, is supplied by HiMedia. It has a pH in the range of 6.8 to 8.2 and an osmolality between 260.00 -340.00 mOsm/KgH2O. It is advisable to store this product between -10 to -40C, away from bright light.

New Born Calf Serum is processed from calves that are 3-10 days old. It contains higher levels of proteins and immunoglobulins and less growth factors than Fetal Bovine Serum (FBS). It is a cost effective alternative to FBS and can be used for culturing most mammalian cell lines. It has proved to be an efficient growth promoter in 3T3 cells (mouse fibroblasts). It is used in fermentation industries where large quantity of serum is required, as an internal reference standard for quantitative protein assays such as double radial immuno-diffusion assay, ELISA, Western immunoblotting, electroimmuno-diffusion, as a blocking agent or as a negative control in nonprecipitating antibody-binding assays and as a reference serum in nephelometry and other automated precipitation techniques.Heat inactivation is done to destroy heat labile components such as complement that can lead to complement mediated cell lysis. Complement proteins, antibodies and enzymes present in the serum are inactivated by heat inactivation.