Antibiotic Susceptibility Tests are conducted in microbiological laboratories to study the effect of an antibiotic on the growth of a microbe. The most common method to study antibiotic susceptibility includes the Kirby-Bauer Method. In this method, small discs saturated with antibiotic is placed on the plate containing the bacteria. A zone of inhibition of growth around the disc is observed to study the susceptibility of the microbe to the antibiotic. Novobiocin-NV-30mcg is a product by Micromaster. It is categorised as an aminocoumarin antibiotic. It is naturally produced by the organism Streptomyces niveus. The drug is found to be active against Staphylococcus epidermidis. The drug exerts its antibiotic activity by blocking the enzyme DNA gyrase. This enzyme inhibits one of the subunits of the enzyme and competitively inhibits the ATPase reaction of the enzyme. The drug also inhibits the DNA nicking and ligation activity of DNA gyrase. Hsp90 is another protein inhibited by Novobiocin. It also binds to and activates the gram-negative lipopolysaccharide transporter LptBFGC.

Specifications:

Product name: Novobiocin-NV-30mcg

Brand: Micromaster

Molecular formula: C31H36N2O11

Molecular mass: 612.624 g/mol