Nutrient Mixture F-10 Ham With L-Glutamine Without Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 6.0 -6.6 whereas the pH with Sodium Bicarbonate is between 7.3 -7.8. The osmolality without Sodium Bicarbonate is in the range of 260.00 -300.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 280.00 -320.00 Osm/Kg H20. It is advisable to store this product between 2-8C. Media composition (in mg/L) is as follows:

Calcium chloride dihydrate (44.10)

Copper sulphate pentahydrate (0.0025)

Ferric sulphate heptahydrate (0.83)

Magnesium sulphate anhydrous (74.73)

Potassium chloride (285.00)

Potassium dihydrogen phosphate (83.00)

Sodium chloride (7400.00)

Sodium phosphate dibasic anhydrous (153.70)

Zinc sulphate heptahydrate (0.028)

Amino acids

Biotin (0.02)

Choline chloride (0.7)

D-Ca-Pantothenate (0.71)

Folic acid (1.32)

Nicotinamide (0.61)

Pyridoxine hydrochloride (0.21)

Riboflavin (0.38)

Thiamine hydrochloride (1.00)

Vitamin B12 (1.36)

i-Inositol (0.54)

D-Glucose (1100.00)

Hypoxanthine Sodium Salt (4.08)

Lipoic acid (0.21)

Phenol red Sodium Salt (1.30)

Sodium pyruvate (110.00)

Thymidine (0.73)

Ham's Nutrient Mixtures were originally developed for single cell plating of near diploid Chinese hamster ovary (CHO) cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured. Ham's Nutrient Mixture F10 was designed for clonal growth of CHO cells and chick embryo cells under serum free conditions. It is now widely used for culturing a variety of cells which include human diploid cells and white blood cells for chromosomal analysis and primary explants of rat, rabbit and chicken tissues.