Nutrient Mixture F-12 Ham,Kaighn's Modification With L-Glutamine Without Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 5.3 -5.9 whereas the pH with Sodium Bicarbonate is between 7.4 -8.0. The osmolality without Sodium Bicarbonate is in the range of 270.00 -310.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 320.00 -360.00 Osm/Kg H20. It is advisable to store this product between 2-8C. Media composition (in mg/L) is as follows:

Calcium chloride dihydrate (135.24)

Copper sulphate pentahydrate (0.0025)

Disodium hydrogen phosphate (115.02)

Ferrous sulphate heptahydrate (0.83)

Magnesium chloride anhydrous (49.70)

Magnesium sulphate anhydrous (93.70)

Potassium chloride (283.29)

Potassium dihydrogen phosphate (58.50)

Sodium chloride (7597.20)

Zinc sulphate heptahydrate (0.14)

Amino acids

Biotin (0.007)

Choline chloride (13.96)

D-Ca-Pantothenate (0.48)

Folic acid (1.32)

Nicotinamide (0.04)

Pyridoxine hydrochloride (0.06)

Riboflavin (0.04)

Thiamine hydrochloride (0.34)

Vitamin B12 (1.36)

i-Inositol (18.00)

D-Glucose (1260.00)

Hypoxanthine Sodium Salt (4.08)

Lipoic acid (0.21)

Phenol red Sodium Salt (3.32)

Putrescine dihydrochloride (0.32)

Sodium pyruvate (220.00)

Thymidine (0.72)

Ham's Nutrient Mixtures were originally developed for single cell plating of near diploid Chinese hamster ovary (CHO) cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured. Ham's Nutrient Mixture F12 was originally designed for serial propagation and cloning of two CHO cell lines namely, CHD-3 and CHL-1 and mouse L cells. It is the medium of choice for the growth of cells of rodent origin and for cloning of myeloma and hybridoma cells. This medium is also the medium of choice for clonal toxicity assay using CHO cells. Kaighn's modification of Ham's F-12 is a complex formulation of F-12 with increased amounts of amino acids and pyruvate. Salts used in this formulation are as given by Konisberg. This modification favors the growth and differentiation of rat and chicken cells and primary human liver cells.