Nutrient Mixture F-12 Ham Without L-Glutamine and Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 5.2 -5.8 whereas the pH with Sodium Bicarbonate is between 7.2 -7.7. The osmolality without Sodium Bicarbonate is in the range of 260.00 -300.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 290.00 -330.00 Osm/Kg H20. It is advisable to store this product between 2-8C. Media composition (in mg/L) is as follows:

Calcium chloride dihydrate (44.10)

Copper sulphate pentahydrate (0.0025)

Ferric sulphate heptahydrate (0.83)

Magnesium chloride anhydrous (57.65)

Potassium chloride (223.60)

Sodium chloride (7599.00)

Sodium phosphate dibasic anhydrous (142.04)

Zinc sulphate tetrahydrate (0.86)

Amino acids

Biotin (0.007) Choline chloride (13.96)

D-Ca-Pantothenate (0.48)

Folic acid (1.32)

Nicotinamide (0.04)

Pyridoxine hydrochloride (0.06)

Riboflavin (0.04)

Thiamine hydrochloride (0.34)

Vitamin B12 (1.36)

i-Inositol (18.00)

D-Glucose (1801.60)

Hypoxanthine Sodium Salt (4.77)

Linoleic acid (0.08)

Lipoic acid (0.21)

Phenol red Sodium Salt (1.24)

Putrescine dihydrochloride (0.16)

Sodium pyruvate (110.10)

Thymidine (0.73)

Ham's Nutrient Mixtures were originally developed for single cell plating of near diploid Chinese hamster ovary (CHO) cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured. Ham's Nutrient Mixture F12 was originally designed for serial propagation and cloning of two CHO cell lines namely, CHD-3 and CHL-1 and mouse L cells. It is the medium of choice for the growth of cells of rodent origin and for cloning of myeloma and hybridoma cells. This medium is also the medium of choice for clonal toxicity assay using CHO cells.