Use Phenogel GPC Columns for Separations of Low, Moderate, and High Molecular Weight:

• Organic-soluble polymers
• Plastics and plasticizers
• Rubbers, resins, and elastomers
• Pharmaceutical excipients
• Food and cosmetic excipients
• Textiles/dyes
• Petroleum and fuels
• Oils, lipids, and fatty acids
• Sample cleanup by GPC

Phenogel Non-Aqueous GPC/SEC Columns

Phenogel packing materials are made from styrene-divinylbenzene by emulsion polymerization under conditions to optimize pore size, total pore volume, particle size, and degree of cross-linking. The results yield Gel Permeation Chromatography (GPC) materials that provide very high resolution, rugged durability, and wide solvent compatibility.

Solvent and Temperature Compatibility

Although styrene-divinylbenzene materials are exceptionally inert, interaction between the column packing and sample materials (especially very polar solutes) occasionally occurs and the mobile phase must be modified to eliminate this effect. Phenogel columns can tolerate up to 0.1% low MW amines, 0.5% glacial acetic acid, or up to 1% water in the mobile phase as modifiers.

Phenogel columns are temperature stable to 140 C which is important for applications involving solutes with limited solubility at ambient temperatures, or where solubility considerations demand the use of viscous solvents such as DMF or DMSO. At higher temperatures, eluent viscosity is decreased and mass transfer is enhanced, with the effect of increasing sample resolution. This temperature stability is particularly useful when analyzing polymers such as polyethylene and polypropylene which require higher temperatures.

Solvent Switching Considerations

Although Phenogel columns are rugged and can withstand strong solvent changes, care should be exercised when switching from high swell solvents (A) to low swell solvents (B, C, and D), see diagram below. Improper solvent switches can result in a void. Best results are attained when an intermediate swell solvent is used, and column lifetime is improved. Contact Phenomenex regarding solvents not listed below. Column life can be maximized by dedicating certain columns to certain solvents. This will also minimize solvent switches. If care is not taken, a void may occur.