Phosphate Buffered Saline (PBS) pH 7.4 is supplied by HiMedia. It is available in the form of a white to cream homogeneous free flowing powder. It is advisable to store this product below 30C. Composition (in gm/L) is as follows:

Sodium chloride (7.65)

Disodium phosphate, Anhydrous (0.72)

Dipotassium hydrogen phosphate (0.21)

Phosphate Buffered Saline (PBS) pH 7.4 is prepared as recommended by BAM. It is generally used as a diluent and sample preparation of Vibrio species. Phosphates maintain the pH of the medium and sodium chloride maintains the osmotic balance. Phosphate-Buffered Saline (PBS), pH 7.4 is used for the preparation of dilution, blanks for the examination of samples from food, water and other specimens.