Ponceau-S Stain is supplied by HiMedia. It is available in the form of a red coloured solution. It binds non-covalently to non-polar regions of a protein. It is also called a negative stain as it binds to the positively charged amino groups of the protein. Ponceau-S Stain is used for the detection of protein bands on cellulose acetate, PVDF and nitrocellulose membranes but it is not suitable for use with nylon membranes. After the protein samples are electro transferred to PVDF or nitrocellulose membranes the staining procedure is carried out and the protein bands are detected with a clear background and red protein bands. This staining technique is completely reversible to allow further immunological detection. It is composed of 0.5% (w/v) Ponceau S in 1% (v/v) glacial acetic acid. It is advisable to store this product at 2-8C.

Ponceau-S Stain is a readytouse, reversible staining solution formulated for fast staining of protein bands on nitrocellulose or PVDF membranes during Western blotting procedure as well as cellulose acetate membranes. After staining the protein bands can be washed off with water and the membrane can be used for immunological detection. Ponceau-S Stain is used for the detection of protein bands on cellulose acetate, PVDF and nitrocellulose membranes. As this staining can be erased of easily it is used for checking of protein bands electro transferred on membrane before proceeding for immunological detection during Western Blotting procedures.