Prestained Protein Ladder is supplied by HiMedia. The Prestained Protein Ladder is a three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weight from 10 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The Pre-Stained Protein Ladder is designed for monitoring protein separation during SDSpolyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. It is advisable to store this product at -20C. Recommended load: 5 l/lane

Prestained Protein Ladder, Broad Range (10-230 kDa) is a mixture of highly purified recombinant proteins covalently coupled to a blue dye that resolves into 12 sharp bands when electrophoresed. The protein concentrations are carefully balanced for even intensity. Both 80 kDa and 25 kDa bands have triple the intensity of other proteins and serve as reference indicators. The covalent coupling of the dye to the proteins affects their electrophoretic behaviour on SDS-PAGE gels relative to unstained proteins.