pUC18 (Cesium Chloride Purified) is provided by Genei for aiding in molecular cloning applications. Plasmids are extrachromosomal, double-stranded DNA , which naturally occur in bacteria. Since plasmids are self-replicating units that can be easily introduced into hosts such as E.coli, plasmids are very useful in recombinant DNA technology. pUC18 is an artificial plasmid, a double-stranded circular DNA of size 2.7 kb. It has a very high copy number and hence is used as an expression vector. pUC18 has the following components:

(g) AmpR: An antibiotic selective marker

(h) Promoter gene: this is a beta-galactosidase inducible promoter

(i) Polylinker region: this region contains restriction sites for several restriction enzymes (HindIII, Pst1, BamHI, Sal1, EcoRI, etc.)

Plasmids find several applications in areas such as recombinant DNA technology, creation of molecular weight markers for gel electrophoresis, sequencing of DNA etc.

This pUC18 from Genei is purified using Caesium chloride density gradient.

Product specifications:

Product name: pUC18 (Cesium Chloride Purified)

Brand: Genei

Quantity: 50 g

Catalogue number: 1600170501730

Storage: -20 C

Price: Rs. 5,880.00