Rappaport Vassiliadis Salmonella Enrichment Broth (As per IP), is supplied by the company, HiMedia.

This is a Rappaport Vassiliadis Salmonella Enrichment Broth used for selective enrichment ofSalmonellaspecies in accordance with Indian Pharmacopoeia.Salmonellausually survive high osmotic pressure, grow at slightly low pH and are resistant to malachite green which inhibits many gram-positive bacteria and concurrently selectively enrich Salmonella. Magnesium chloride is present in the broth which contributes in the rise of the osmotic pressure. Soya peptone enhance the growth ofSalmonella.NaCl in the medium maintains the osmotic balance.

Product specifications:

• Catalog number: LQ104l-50X10ML
• Brand: HiMedia
• Quantity: Pack of 50X10ML

Product composition:

• Soya peptone: 4.500 gm/L
• Sodium chloride: 8.000 gm/L
• Dipotassium phosphate: 0.400 gm/L
• Potassium dihydrogen phosphate: 0.600 gm/L
• Magnesium chloride hexahydrate: 29.000 gm/L
• Malachite green: 0.036 gm/L

Product features:

• Appearance:Rappaport Vasiliadis Salmonella Enrichment Broth (MM1491)
• Colour: Bluish green coloured solution
• Quantity of medium: 10 ml of medium in bottles
• pH: 5.00- 5.40
• Culture response:Cultural characteristics observed after incubation at 35 - 37C for 18 - 24 hours
• Sterility method: Sterilized by autoclaving at 115 C for 30 minutes
• Sterility Assurance Level Sterility assurance level of media was validated against B.subtilis Spore strips (LA924). The spore strips exposed at 115C and unexposed strips were inoculated seperately in 100ml Soyabean Casein Digest Medium and incubated at 35C for 7 days. No growth observed Luxuriant growth observed
• Sterility test:Passes release criteria
• Storage and shelf-life:Store between 2-8C. Use before expiry date on the label.
• Culture response:Cultural characteristics observed after incubation at 30-35C for18-24hours. Recovery is carried out using XLD Agar (MM031)