RPMI- 1640 w/ 25mM HEPES buffer and Sodium pyruvate w/o L-Glutamine and NaHCO3
- Brand: HIMEDIA
- Catalog No.: AT157A-20L
- Quantity/Unit: 20lt/EA
- Usually Shipped in: 2-3 Weeks
Min Orderable Qty : 1 Pack
For lab/research use only, unless otherwise specified
RPMI-1640 With HEPES Buffer and Sodium pyruvate Without L-Glutamine and Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 6.4 -7.0 whereas the pH with Sodium Bicarbonate is between 6.7 -7.3. The osmolality without Sodium Bicarbonate is in the range of 290.00 -330.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 330.00 -370.00 Osm/Kg H20. It is advisable to store this product between 2-8C. Media composition (in mg/L) is as follows:
Calcium nitrate tetrahydrate (100.00)
Disodium hydrogen phosphate anhydrous (800.00)
Magnesium sulphate anhydrous (48.84)
Potassium chloride (400.00)
Sodium chloride (6000.00)
Amino Acids
Choline chloride (3.00)
D-Biotin (0.20)
D-Ca-Pantothenate (0.25)
Folic acid (1.00)
Niacinamide (1.00)
Pyridoxine hydrochloride (1.00)
Riboflavin (0.20)
Thiamine hydrochloride (1.00)
Vitamin B12 (0.005)
i-Inositol (35.00)
p-Amino benzoic acid (PABA) (1.00)
D-Glucose (2000.00)
Glutathione reduced (1.00)
HEPES Buffer (5958.00)
Phenol Red Sodium Salt (5.30)
Sodium Pyruvate (110.00)
Roswell Park Memorial Institute (RPMI) media are a series of media developed by Moore et al for the culture of human normal and neoplastic cells in vitro. RPMI 1640 is the most commonly used medium in the series. A modification of McCoy's 5A medium, the medium was specifically designed to support the growth of human lymphoblastoid cells in suspension culture. Presently the medium is extensively used for a wide range of anchorage dependant cell lines. The medium needs to be supplemented with 5-20