RPMI-1640 w/ Sodium pyruvatE w/o L-Glutamine, Phenol red and Sodium bicarbonate-AT229A-10X1L
- Brand: HIMEDIA
- Catalog No.: AT229A-10X1L
- Quantity/Unit: 10x1lt/EA
- Usually Shipped in: 2-3 Weeks
Min Orderable Qty : 1 Pack
For lab/research use only, unless otherwise specified
RPMI 1640 with Sodium Pyruvate Without L-Glutamine, Phenol red and Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. The pH without Sodium Bicarbonate is between 7.3 -7.9 whereas the pH with Sodium Bicarbonate is between 7.6 -8.2. The osmolality without Sodium Bicarbonate is in the range of 230.00 -270.00 Osm/Kg H20 whereas the osmolality with Sodium Bicarbonate 280.00 -320.00 Osm/Kg H20. It is advisable to store this product between 2-8C. Media composition (in mg/L) is as follows:
Calcium nitrate tetrahydrate (100.00)
Disodium hydrogen phosphate anhydrous (800.00)
Magnesium sulphate anhydrous (48.84)
Potassium chloride (400.00)
Sodium chloride (6000.00)
Amino acids
Choline chloride (3.00)
D-Biotin (0.20)
D-Ca-Pantothenate (0.25)
Niacinamide (1.00)
Pyridoxine hydrochloride (1.00)
Riboflavin (0.20)
Thiamine hydrochloride (1.00)
Vitamin B12 (0.005)
i-Inositol (35.00)
p-Amino benzoic acid (PABA) (1.00)
D-Glucose (2000.00)
Glutathione reduced (1.00)
Roswell Park Memorial Institute (RPMI) media are a series of media developed by Moore et al for the culture of human normal and neoplastic cells in vitro. RPMI 1640 is the most commonly used medium in the series. A modification of McCoy's 5A medium, the medium was specifically designed to support the growth of human lymphoblastoid cells in suspension culture. Presently the medium is extensively used for a wide range of anchorage dependant cell lines. The medium needs to be supplemented with 5-20