MBRE012-250U is referred as Sac I,under HiMedia catalogue. MBRE012 Sac I restriction endonuclease enzyme is isolated from Streptomyces achromogenes. Restriction enzymes are DNA-cutting enzymes found in bacteria. They identify and cut DNA only at a particular sequence of nucleotides. Restriction enzymes are applicable in molecular biology
Specifications:
Source: E.coli strain that carries the Sac I gene from Streptomyces achromogenes
Concentration: 40 U/l
Reagents provided: Sac I , 10X HiBuffer H 2 ,10X HiBuffer DB,Diluent E Buffer
Applications: Molecular biology
Storage conditions: Sac I should be stored at -20C
Pack size: 250 units (1 u is defined as the amount of enzyme that is required to digest 1g of DNA in 1 hour at 37C in 50l of assay buffer.