Soyabean Casein Digest Agar Plate with 0.1% Polysorbate 80 (g-irradiated) is supplied by HiMedia. It contains 25ml of sterile ready prepared media in 90mm disposable plates. This light to medium amber coloured product has a pH in the range of 7.1 to 7.5. Soyabean Casein Digest Agar is used for cultivation of wide variety of microorganisms.It is advisable to store this product between 15-25C. Composition (in gm/L) is as follows:

Pancreatic digest of casein (15.00)

Papaic digest of soyabean meal (5.00)

Sodium chloride (5.00)

Agar (15.00)

Lecithin (0.70)

Polysorbate 80 (Tween 80) (5.00)

Histidine (0.50)

Sodium thiosulphate (0.50)

Soyabean Casein Digest Agar is generally used for the detection and enumeration of microorganisms for products of sanitary importance, water miscible cosmetics, products containing antimicrobials or preservatives. Casein enzymic hydrolysate and papaic digest of soyabean meal supply nitrogenous compounds and other nutrients which are essential for microbial replication. Lecithin, polysorbate 80 (Tween 80) and thiosulphate neutralize the activity of antimicrobial agents. They neutralize quaternary ammonium compounds and parahydroxy benzoates too. Sodium thiosulphate can neutralize mercurial compounds, halogens, aldehydes etc. Histidine acts as a reducing agent. Testing samples from areas before and after the treatment with disinfectant can be used to evaluate cleaning procedures in environmental sanitation. The presence and number of microorganisms is determined by the appearance of colonies on the agar surface.