Soyabean Casein Digest Agar Plate w/ LTHTh is supplied by HiMedia. It contains 25ml of sterile Soyabean Casein Digest Agar in 90mm disposable plates. This light to medium amber coloured product has a pH in the range of 7.1 to 7.5. Soyabean Casein Digest Agar is used for determining the efficiency of sanitization of containers, equipment surfaces, water miscible cosmetics, etc. It can also be used to enumerate the organisms from water insoluble products and fatty products containing preservatives or antimicrobials. It is advisable to store this product between 15-25C. Composition (in gm/L) is as follows:

Pancreatic digest of casein (15.00)

Papaic digest of soyabean meal (5.00)

Sodium chloride (5.00)

Agar (15.00)

Lecithin (0.70)

Polysorbate 80 (Tween 80) (5.00)

Histidine (0.50)

Sodium thiosulphate (0.50)

Soyabean Casein Digest Agar w/ LTHTh is used for the detection and enumeration of microorganisms for products of sanitary importance, water miscible cosmetics, Products containing antimicrobials or preservatives. Casein enzymic hydrolysate and papaic digest of soyabean meal provide nitrogenous compounds and other nutrients essential for microbial replication. Lecithin, polysorbate 80 (Tween 80) and thiosulphate act as neutralizing agents reported to neutralize the activity of antimicrobial agents. Lecithin and polysorbate 80 neutralizes quaternary ammonium compounds and parahydroxy benzoates. Sodium thiosulphate neutralizes mercurial, halogens, aldehydes etc. Histidine acts as a reducing agent. Collection of samples from areas before and after the treatment with disinfectant evaluates cleaning procedures in environmental sanitation. The presence and number of microorganisms is determined by the appearance of colonies on the agar surface.