MBRE014-5000U is referred as Sph I,under HiMedia catalogue. MBRE014 Sph I restriction endonuclease enzyme is isolated from Streptomyces phaeochromogenes. Restriction enzymes are DNA-cutting enzymes found in bacteria. They identify and cut DNA only at a particular sequence of nucleotides. Restriction enzymes are applicable in molecular biology
Specifications:
Source: E.coli strain that carries the Sph I gene from Streptomyces phaeochromogenes
Concentration: 5 U/l
Reagents provided: Sph I ,10X HiBuffer DB,Diluent E Buffer
Applications: Molecular biology
Storage conditions: Sph I should be stored at -20C
Pack size: 5000 units (1 u is defined as the amount of enzyme that is required to digest 1g of DNA in 1 hour at 37C in 50l of assay buffer.