Soil samples are complex environments characterized by the presence of inhibitory compounds, such as humic acid, heavy metals, and other aromatic components which may prove to be challenging for downstream analyses. The SPINeasy DNA Pro Kit for Soil has been carefully designed for the isolation of pure microbiome genomic DNA from challenging soil types including those with low biomass or those highly contaminated. The SPINeasy DNA Pro Kit for Soil effectively lyses various microbiome population, including bacteria, fungi, viruses, and protists. The kit provides similar yields to that of our highly cited FastDNA SPIN Kit, but with improved purity and reduced processing time. Isolated DNA products showed no inhibition in PCR and were immediately ready to be used for downstream applications, including long fragment PCR, qPCR, and next-generation sequencing (16S and whole genome) without the need of further inhibitor removal step.