SPINeasy Plasmid Midiprep Kit is a silica-membrane spin-column kit for the purification of high-copy and low-copy number plasmid DNA from 25 50 mL of transformed bacterial culture. Using our specially formulated buffers, bacterial cells are disrupted by alkaline lysis to release plasmid DNA. Cell debris, such as chromosomal DNA and proteins, are precipitated by neutralization and cleared through centrifugation. Subsequently, plasmid DNA in the lysate supernatant is adsorbed on a silica membrane, washed and eluted in a convenient process using the spin column technology. Up to 1 mg of plasmid DNA can be purified through this simple workflow, which is immediately ready for routine molecular biology laboratory applications.