To be used in conjunction with Caymans SREBP-2 polyclonal antibody (Catalog No. 10007663) to block protein-antibody complex formation during immunochemical analysis of SREBP-2 protein. SREBPs, including SREBP-1a, SREBP-1c, and SREBP-2, constitute a family of basic helix-loop-helix (bHLH) transcription factors that play a critical role in lipid homeostasis by regulating genes involved in cholesterol and fatty acid metabolism.1 EAch SREBP consists of three domains, including an amino-terminal transcription factor domain of ~480 amino acids, a middle hydrophobic region of ~80 amino acids containing two hydrophobic transmembrane segments, and a carboxy-terminal regulatory domain of ~590 amino acids.1 SREBP-2 regulates cholesterol synthesis by activating the transcription of genes for HMG-CoA reductase and other enzymes of the cholesterol synthetic pathway.2 SREBP-2 is ubiquitously detected in various tissues.3 Under basal conditions SREBP is bound to ER membranes as a glycosylated precursor protein. Upon cholesterol depletion, the protein is clEAved to its active forms (about 50-68 kDa) and translocated into the nucleus to stimulate transcription of genes involved in the uptake and synthesis of cholesterol.4 Caymans SREBP-2 polyclonal antibody detects both precursor and active forms of the protein in tissues and cells such as liver, brown fat, testis, HepG2 cells, and human fibroblast. The apparent molecular weight on SDS-PAGE may be higher than the calculated molecular weight (about 126 kDa) due to glycosylation of the protein.5 Technical Information Synonyms SREBF2 Sterol Regulatory Element-binding Protein-2 Sterol Regulatory Element-binding Transcription Factor 2 Amino Acids human SREBP-2 protein amino acids 455-469 (SPLLDDAKVKDEPDS) Formulation 200 ug of peptide in 200 l TBS, pH 7.4, containing 0.1% BSA and 0.02% sodium azide