High grade Staphylococcus Agar No.110 (Gelatin Mannitol Salt Agar) is supplied by Micromaster. It is available in the form of a cream to yellow homogenous free flowing powder with a pH in the range of 6.8 to 7.2. It is advisable to store this product below 30C.

• Quantity provided: 100 g/pack

Staphylococcus Agar No.110 is used as a selective medium for the isolation and testing of pathogenic Staphylococci. It is also known as Stone Gelatin Agar and is used for the selective isolation based on few characteristic properties of pathogenic Staphylococci like, pigment production, mannitol fermentation and gelatin liquefaction. Staphylococcus Agar No. 110 is recommended by APHA and AOAC. The medium can be used with Egg Yolk Emulsion to study the egg yolk reactions. Casein enzymic hydrolysate and yeast extract serve as sources of carbon, nitrogen and other essential nutrients and growth factors including vitamins. D-Mannitol is the fermentable carbohydrate with lactose being an additional source of carbon. Sodium chloride maintains the osmotic equilibrium while phosphate buffers the medium. Gelatin serves as the substrate for gelatin liquefaction. Mannitol fermentation can be visualized as yellow colouration by addition of a few drops of bromothymol blue to the areas of the plates where colonies have been removed. Gelatin liquefaction can be seen when the plates are flooded with a saturated aqueous solution of ammonium sulphate. On incubation at 35-37C for 10 minutes, a clear zone can be observed. Enterococcus faecalis may grow on this medium as small colonies with slight mannitol fermentation.