Auras TMB substrate is a chromagen substrate, 3,3,5,5-tetramethylbenzidine (TMB) provided in a mildly acidic, non-hazardous buffer for horseradish peroxidase detection in an ELISA format. The substrate is provided as a ready-to-use working dilution in a slightly acidic, non-hazardous proprietary buffer without organic solvents. The substrate develops a blue reaction product when oxidized by peroxidase and a yellow reaction product in an endpoint in assay after the addition of an acid solution provided by the end-user. This TMB substrate is stable for 24 months at 4C, providing an extended shelf-life; the assay end product is stable for at least one hour after stopping the assay.

Advantages

Ready to use single reagent

Free of Toxic Preservatives

Free of Harmful Organic Solvents

More stable

More sensitivity, stability and stop-stability