X-Press Blue Protein Stain (SafeBlue Protein Stain) is supplied by HiMedia. X-Press Blue Protein Stain (SafeBlue Protein Stain) is Coomassie G-250 based rapid, ultrasensitive ready-to-use staining solution used in visualizing protein bands on SDS-PAGE. Unlike other gel staining systems, no destaining is required while using this stain. It is advisable to store this product between 15-25C.

X-Press Blue Protein Stain (SafeBlue Protein Stain) is a ready-to-use solution containing Coomassie Brilliant Blue G-250 at a low pH and can detect 200 ng of protein. This protein staining reagent has higher sensitivity and gives lower background compared to the conventional Coomassie stains. This ready to use reagent develops visible protein bands directly in the staining tray. Thus, usage of this solution saves a lot of time required for solution preparation. After staining, an optional water-wash step further enhances staining sensitivity and yields a very low background. With this reagent there is no need for multistep de-staining procedures. This reagent stains only proteins and leaves the gel background relatively unstained so that the protein bands are visualized directly during the staining process. This results in the saving of time as the staining can be stopped when the protein bands have been properly stained. Although a conventional de-staining step with methanol and acetic acid is not required as with other Coomassie-based stains, rinsing the stained gel with water enhances the sensitivity.